7KJA
Crystal structure of the EphA2 intracellular KD-SAM domains
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E SUPERBRIGHT |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2014-11-21 |
| Detector | RIGAKU RAXIS HTC |
| Wavelength(s) | 1.5418 |
| Spacegroup name | I 1 2 1 |
| Unit cell lengths | 123.060, 55.030, 135.644 |
| Unit cell angles | 90.00, 94.86, 90.00 |
Refinement procedure
| Resolution | 20.490 - 1.750 |
| R-factor | 0.1898 |
| Rwork | 0.188 |
| R-free | 0.21750 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4pdo 3kka |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.715 |
| Data reduction software | MOSFLM (version 7.1.0) |
| Data scaling software | Aimless (version 0.3.11) |
| Phasing software | PHASER (2.5.6) |
| Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.490 | 1.780 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.059 | 0.690 |
| Rmeas | 0.081 | 0.953 |
| Rpim | 0.056 | 0.654 |
| Number of reflections | 91439 | 4522 |
| <I/σ(I)> | 13.4 | 1.4 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 3.6 | 3.5 |
| CC(1/2) | 0.997 | 0.599 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 287 | 0.2 M MgCl2, 0.1 M Bis-Tris pH5.5, 25% PEG 3350 |
