7KIJ

Muscovy duck circovirus Rep domain complexed with a single-stranded DNA 10-mer comprising the cleavage site

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-09-27
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.97910
Spacegroup name	P 61 2 2
Unit cell lengths	102.986, 102.986, 138.294
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	54.650 - 1.690
R-factor	0.1718
Rwork	0.171
R-free	0.19460
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6wdz
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	54.650	1.751
High resolution limit [Å]	1.690	1.690
Rmerge	0.036	0.825
Rmeas	0.039	0.878
Rpim	0.013	0.292
Number of reflections	48935	4791
<I/σ(I)>	32.89	2.28
Completeness [%]	99.8	99.69
Redundancy	8.7	8.5
CC(1/2)	1.000	0.784

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.6	298	0.1 M sodium acetate, pH 4.8, 2.2M ammonium sulfate, 20% glycerol added to drop