7KI3

Human Argonaute2:miR-122 bound to the HCV genotype 1a site-1 RNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	80
Detector technology	PIXEL
Collection date	2017-09-07
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1
Spacegroup name	P 21 21 21
Unit cell lengths	87.313, 112.963, 207.684
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	99.234 - 3.000
R-factor	0.2349
Rwork	0.233
R-free	0.27600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4W5R MID and PIWI domains
Data reduction software	DIALS (2)
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.14rc1_3161)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	99.234	3.107
High resolution limit [Å]	3.000	3.000
Number of reflections	58923	50608
<I/σ(I)>	12.52	1.58
Completeness [%]	100.0
Redundancy	12.6
CC(1/2)	0.885	0.537

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	293	PEG8000, barium chloride, Tris-HCl