7KFW
Structural basis for a germline-biased antibody response to SARS-CoV-2 (RBD:C1A-B3 Fab)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2020-07-25 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 84.174, 112.843, 267.846 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 133.920 - 2.792 |
| R-factor | 0.1913 |
| Rwork | 0.189 |
| R-free | 0.22930 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7kfv |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.980 |
| Data reduction software | XDS (20200131) |
| Data scaling software | XDS (20200131) |
| Phasing software | PHASER (2.8.3) |
| Refinement software | BUSTER (2.10.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 200.000 | 2.940 |
| High resolution limit [Å] | 2.770 | 2.790 |
| Rmeas | 0.382 | |
| Number of reflections | 63645 | 17922 |
| <I/σ(I)> | 5.41 | 0.68 |
| Completeness [%] | 98.1 | 88.6 |
| Redundancy | 6.94 | 6.51 |
| CC(1/2) | 0.983 | 0.233 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | protein sample: 13 mg/mL in 150mM NaCl, 25 mM Tris-HCl, pH 7.5 mother liquor composition (equal volume): 0.2 M Ammonium phosphate dibasic, 20% w/v PEG 3350 |
