7KFN
Structure of Human Adenosine Deaminase Acting on dsRNA (ADAR2) bound to dsRNA containing a 2'-deoxy Benner's Base Z opposite the edited base
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-07-24 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.97918 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 172.024, 63.612, 132.149 |
| Unit cell angles | 90.00, 126.77, 90.00 |
Refinement procedure
| Resolution | 68.990 - 2.500 |
| R-factor | 0.1733 |
| Rwork | 0.171 |
| R-free | 0.22560 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6d06 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.580 |
| Data reduction software | Aimless |
| Data scaling software | Aimless (0.7.4) |
| Phasing software | PHASER (2.7.18) |
| Refinement software | REFMAC (5.8.0253) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 105.860 | 105.860 | 2.600 |
| High resolution limit [Å] | 2.500 | 9.010 | 2.500 |
| Rmerge | 0.110 | 0.050 | 0.613 |
| Rmeas | 0.127 | 0.058 | 0.714 |
| Rpim | 0.063 | 0.029 | 0.360 |
| Total number of observations | 152256 | 3325 | 16610 |
| Number of reflections | 39692 | 908 | 4422 |
| <I/σ(I)> | 8.7 | 20.3 | 2.2 |
| Completeness [%] | 99.4 | 99.4 | 99.1 |
| Redundancy | 3.8 | 3.7 | 3.8 |
| CC(1/2) | 0.993 | 0.995 | 0.714 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 298 | 100 mM MES:NaOH pH 6.5, 18% PEG 20,000 |
