7KFK

Crystal structure of LILRB1 D3D4 domain in complex with Plasmodium RIFIN (PF3D7_1373400) V2 domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-09-25
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1
Spacegroup name	P 42 21 2
Unit cell lengths	111.044, 111.044, 152.124
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.440 - 2.630
R-factor	0.2291
Rwork	0.227
R-free	0.27080
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4lla
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (dev_3965)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.700
High resolution limit [Å]	2.630	7.190	2.650
Rmerge	0.202	0.070	1.306
Rmeas	0.207	0.073	1.346
Rpim	0.045	0.019	0.322
Number of reflections	28673	1551	1410
<I/σ(I)>	5.2
Completeness [%]	99.8	96.2	99.9
Redundancy	21.2	14.5	16.9
CC(1/2)		0.998	0.896

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.1M Tris 8.3, 11% PEG3350, 1.1mM AmSO4, 0.2mM Li2SO4