7KEE

RNA polymerase II elongation complex with unnatural base dTPT3, rNaMTP bound to E-site

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.3
Synchrotron site	ALS
Beamline	5.0.3
Temperature [K]	100
Detector technology	CCD
Collection date	2019-03-23
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.0
Spacegroup name	C 1 2 1
Unit cell lengths	169.544, 222.739, 194.661
Unit cell angles	90.00, 101.90, 90.00

Refinement procedure

Resolution	49.150 - 3.450
R-factor	0.2595
Rwork	0.259
R-free	0.30190
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2e2j
Data reduction software	XDS
Data scaling software	Aimless (0.7.3)
Phasing software	PHASER
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	49.155	49.150	3.470
High resolution limit [Å]	3.410	18.680	3.410
Rmerge	0.657	0.047	3.320
Rmeas	0.710	0.051	3.596
Rpim	0.267	0.019	1.369
Total number of observations		4217	32004
Number of reflections	95996	584	4693
<I/σ(I)>	4.1	34.4	0.7
Completeness [%]	99.9	94.7	100
Redundancy	7	7.2	6.8
CC(1/2)	0.948	0.998	0.241

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	298	390mM (NH4)2HPO4/NaH2PO4, pH 6.5, 5mM dioxane, 5mM DTT, 10-12% PEG6000