7KD7

Crystal structure of human NatD (NAA40) bound to a bisubstrate analogue

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-E
Synchrotron site	APS
Beamline	21-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-09-26
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9792
Spacegroup name	P 21 21 21
Unit cell lengths	46.433, 74.349, 126.821
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	63.410 - 1.440
R-factor	0.1625
Rwork	0.161
R-free	0.18290
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4u9w
RMSD bond length	0.018
RMSD bond angle	1.587
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	126.820	1.460
High resolution limit [Å]	1.440	1.440
Rmerge	0.041	0.713
Rmeas	0.045
Number of reflections	79927	3775
<I/σ(I)>	23.1	2.5
Completeness [%]	99.4
Redundancy	6.4
CC(1/2)	0.999	0.779

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	293.15	100 mM BIS-TRIS pH 5.5, 2000 mM Ammonium sulfate