7JZ4

Crystal structure of broadly Plasmodium RIFIN reactive LAIR1-inserted antibody MGD21

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-12-20
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1
Spacegroup name	P 1 21 1
Unit cell lengths	80.932, 81.099, 111.561
Unit cell angles	90.00, 94.03, 90.00

Refinement procedure

Resolution	38.933 - 2.747
R-factor	0.1966
Rwork	0.194
R-free	0.24930
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3kgr
RMSD bond length	0.006
RMSD bond angle	0.921
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.800
High resolution limit [Å]	2.747	7.460	2.747
Rmerge	0.103	0.045	0.872
Rmeas	0.120	0.054	1.015
Rpim	0.061	0.028	0.517
Number of reflections	37579	1942	1893
<I/σ(I)>	10.3
Completeness [%]	99.9	99.3	99.5
Redundancy	3.8	3.6	3.8
CC(1/2)		0.996	0.641

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.1 M MgCl2, 0.1 M Tris HCl pH 8.5, 20% PEG 3350, 25% PEG 400