7JYE
Human Liver Receptor Homolog-1 in Complex with 9ChoP and a Fragment of Tif2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2020-02-07 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 89.240, 89.240, 106.537 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 35.512 - 2.551 |
| R-factor | 0.1776 |
| Rwork | 0.159 |
| R-free | 0.18710 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7jyd |
| RMSD bond length | 0.001 |
| RMSD bond angle | 0.320 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.12-2829-000) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.640 |
| High resolution limit [Å] | 2.550 | 5.490 | 2.550 |
| Rmerge | 0.265 | 0.171 | 1.955 |
| Rmeas | 0.276 | 0.178 | 2.030 |
| Rpim | 0.074 | 0.048 | 0.542 |
| Total number of observations | 226693 | ||
| Number of reflections | 16458 | 1765 | 1613 |
| <I/σ(I)> | 3.6 | ||
| Completeness [%] | 100.0 | 99.8 | 100 |
| Redundancy | 13.8 | 13.1 | 13.8 |
| CC(1/2) | 0.987 | 0.571 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 277 | 9-19% tert-butanol, 0-6 % glycerol, 0.1 M tri-sodium citrate pH 4.6 |
