7JV7
Crystal Structure of the yeast RNA Pol II CTD kinase CTDK-1 complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-D |
| Synchrotron site | APS |
| Beamline | 21-ID-D |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2018-10-19 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.004 |
| Spacegroup name | P 1 |
| Unit cell lengths | 55.170, 71.091, 84.797 |
| Unit cell angles | 79.08, 86.22, 72.69 |
Refinement procedure
| Resolution | 29.242 - 1.851 |
| R-factor | 0.184364153002 |
| Rwork | 0.184 |
| R-free | 0.22173 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.865 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.880 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.068 | 0.530 |
| Number of reflections | 91889 | 4321 |
| <I/σ(I)> | 15.6 | |
| Completeness [%] | 89.4 | |
| Redundancy | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.3 | 293 | 0.1 M Sodium Citrate pH 5.3, 0.3 M Lithium Chloride, and 17.5% PEG3350 |
