7JTX

the structural basis of PTEN regulation by multi-site phosphorylation

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-06-13
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9798
Spacegroup name	I 4
Unit cell lengths	111.865, 111.865, 60.656
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.550 - 3.230
R-factor	0.2316
Rwork	0.228
R-free	0.26290
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1d5r
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.19.1_4122)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	56.010	3.290
High resolution limit [Å]	3.050	3.050
Rmerge	0.145	1.561
Rpim		0.893
Number of reflections	6977	10
<I/σ(I)>	9.2
Completeness [%]	98.7
Redundancy	4.5
CC(1/2)	0.993

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	300 mM sodium cistrate tribasic, 20% PEG 3350