7JTF

Structure of Hepatitis C Virus Envelope Glycoprotein E2 core from genotype 6a bound to broadly neutralizing antibody RM2-01

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-04-06
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	1.0332
Spacegroup name	P 1 21 1
Unit cell lengths	89.732, 64.887, 145.208
Unit cell angles	90.00, 105.16, 90.00

Refinement procedure

Resolution	29.860 - 3.350
R-factor	0.2449
Rwork	0.242
R-free	0.29590
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6bkb
RMSD bond length	0.002
RMSD bond angle	0.449
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	3.410
High resolution limit [Å]	3.350	3.350
Number of reflections	21566	631
<I/σ(I)>	16.2
Completeness [%]	91.5
Redundancy	4.8
CC(1/2)	0.960	0.900

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.6	293	30% PEG 400, 0.1M Cadmium chloride, 0.1M sodium acetate, pH 4.6