7JQL

Crystal structure of the Thermus thermophilus 70S ribosome in complex with Bac7-001, mRNA, and deacylated P-site tRNA at 3.00A resolution

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-12-15
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97918
Spacegroup name	P 21 21 21
Unit cell lengths	209.090, 449.880, 621.270
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	76.760 - 3.000
R-factor	0.2286
Rwork	0.226
R-free	0.28550
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4y4p
RMSD bond length	0.004
RMSD bond angle	0.844
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.8.2)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	76.760	76.760	3.080
High resolution limit [Å]	3.000	13.420	3.000
Rmerge	0.262	0.048	1.518
Rmeas	0.293	0.053	1.697
Total number of observations	5833725
Number of reflections	1148853	13415	84775
<I/σ(I)>	5.38	20.87	1.01
Completeness [%]	99.5	97.6	99.7
Redundancy	5.078	5.088	5.034
CC(1/2)	0.991	0.996	0.178

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.6	292	0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME