7JOZ

Crystal structure of dopamine D1 receptor in complex with G protein and a non-catechol agonist

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	80
Detector technology	PIXEL
Collection date	2015-10-01
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.0332
Spacegroup name	P 21 2 21
Unit cell lengths	99.194, 144.305, 147.069
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.000 - 3.800
R-factor	0.2559
Rwork	0.254
R-free	0.29680
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3sn6
RMSD bond length	0.002
RMSD bond angle	0.525
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.000	4.160
High resolution limit [Å]	3.790	3.790
Rmerge	0.189	0.904
Rpim	0.116	0.555
Number of reflections	20110	4783
<I/σ(I)>	4.7
Completeness [%]	94.3	95.2
Redundancy	3.6	3.7
CC(1/2)	0.990	0.468

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	LIPIDIC CUBIC PHASE	5.5	293	0.1M MES pH 6.5, 0.1M Ammonium Dibasic Citrate, 18-25% (w/w) PEG400, 1mM TCEP, 10uM UCB1575401