7JLG

Human PrimPol extending from the erroneous primer base A opposite the 8-oxoguanine lesion

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-06-12
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	1.03324
Spacegroup name	P 1
Unit cell lengths	51.031, 65.210, 73.115
Unit cell angles	71.36, 84.31, 88.63

Refinement procedure

Resolution	26.810 - 2.070
R-factor	0.2016
Rwork	0.201
R-free	0.21880
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5l2x
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	27.841	2.130
High resolution limit [Å]	2.070	2.070
Rmerge	0.069
Number of reflections	52599	4069
<I/σ(I)>	8.8
Completeness [%]	97.0
Redundancy	3.4
CC(1/2)	0.998	0.336

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	225-250 mM CaCl2, 16-19% PEG 3350