7JL4

Crystal structure of TRIM65 PSpry domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-10-14
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.9201
Spacegroup name	P 32
Unit cell lengths	76.269, 76.269, 82.720
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	38.130 - 1.920
R-factor	0.1695
Rwork	0.168
R-free	0.20870
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6fln
RMSD bond length	0.009
RMSD bond angle	1.612
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.18.2-3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.130	1.990
High resolution limit [Å]	1.920	1.920
Number of reflections	41070	4050
<I/σ(I)>	13.34
Completeness [%]	99.9
Redundancy	5.3
CC(1/2)	0.996	0.952

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	PEG3350, Bicine, pH 9.0