7JJL

Crystal structure of Importin Alpha 3 in complex with human LSD1 NLS

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-12-07
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9357
Spacegroup name	P 21 21 21
Unit cell lengths	47.920, 89.120, 143.530
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.310 - 2.600
R-factor	0.2071
Rwork	0.204
R-free	0.26610
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6bvz
Data reduction software	Aimless
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (v1.12)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	33.310	2.600
High resolution limit [Å]	2.600	2.600
Rmerge		0.493
Number of reflections	19536	2323
<I/σ(I)>	2.1
Completeness [%]	99.5
Redundancy	3.7
CC(1/2)	0.997	0.778

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		295	0.7 M sodium citrate, 0.01M DTT, 0.1M sodium HEPES pH 7