7JFL

Crystal structure of human phosphorylated IRF-3 bound to CBP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.1
Synchrotron site	ALS
Beamline	5.0.1
Temperature [K]	120
Detector technology	CCD
Collection date	2016-09-01
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.0
Spacegroup name	C 1 2 1
Unit cell lengths	124.010, 68.030, 55.920
Unit cell angles	90.00, 106.24, 90.00

Refinement procedure

Resolution	46.930 - 1.680
R-factor	0.1927
Rwork	0.191
R-free	0.22290
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5jem
Data reduction software	MOSFLM (1.18.2_3874)
Data scaling software	Aimless (0.5.21)
Phasing software	PHASER
Refinement software	PHENIX (1.17_3644)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.930	1.700
High resolution limit [Å]	1.680	1.680
Rmerge	0.109
Rpim	0.068
Number of reflections	48116	2336
<I/σ(I)>	6.5
Completeness [%]	94.7	90.6
Redundancy	3.4	3.1
CC(1/2)	0.990	0.352

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5	277	0.1 M sodium acetate pH 5.0, 0.2 M MgCl2, 5% PEG 3350