7FGL
The complex structure of PHF core domain peptide of tau, VQIVYK, and antibody's Fab domain.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU FR-E |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2019-08-22 |
Detector | RIGAKU RAXIS VII |
Wavelength(s) | 1.5406 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 59.980, 70.720, 118.030 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 59.080 - 2.100 |
R-factor | 0.20317 |
Rwork | 0.200 |
R-free | 0.26962 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6lra |
RMSD bond length | 0.007 |
RMSD bond angle | 1.539 |
Data reduction software | CrystalClear |
Data scaling software | CrystalClear |
Phasing software | BALBES |
Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 59.080 | 1.970 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.060 | 0.364 |
Number of reflections | 28351 | 28351 |
<I/σ(I)> | 10.6 | |
Completeness [%] | 99.4 | |
Redundancy | 3.47 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 19% PEG3350, 20% Glycerol, 0.2M Ammonium iodide, 0.01M Tris-HCl |