7FAK
Co-crystal Structure of Toxoplasma gondii Prolyl tRNA Synthetase (TgPRS) in complex with L96 and L-pro
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-04-28 |
| Detector | DECTRIS EIGER2 XE 16M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 113.619, 74.230, 74.648 |
| Unit cell angles | 90.00, 111.05, 90.00 |
Refinement procedure
| Resolution | 60.811 - 1.899 |
| R-factor | 0.1829 |
| Rwork | 0.181 |
| R-free | 0.21640 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6aa0 |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.309 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.15) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 60.811 | 1.940 |
| High resolution limit [Å] | 1.890 | 1.890 |
| Rmeas | 0.125 | 0.890 |
| Number of reflections | 45141 | 2157 |
| <I/σ(I)> | 8.7 | 1.3 |
| Completeness [%] | 98.6 | 94.9 |
| Redundancy | 6.6 | 5.7 |
| CC(1/2) | 1.000 | 0.500 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | Morpheus G2 |
