7FAJ
CARM1 bound with compound 43
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-11-22 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97915 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 75.296, 99.339, 207.141 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 60.925 - 2.245 |
| R-factor | 0.193692820483 |
| Rwork | 0.191 |
| R-free | 0.23982 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6izq |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.863 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.12_2829) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 75.300 | 2.300 |
| High resolution limit [Å] | 2.245 | 2.250 |
| Rmerge | 0.074 | 0.656 |
| Number of reflections | 74529 | 74529 |
| <I/σ(I)> | 14.5 | |
| Completeness [%] | 99.3 | 99.9 |
| Redundancy | 13.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | 22-27% PEG 3350, 0.15M sodium malate, pH 7.0 |
