7F3M

Crystal structure of FGFR4 kinase domain with PRN1371

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	Cu FINE FOCUS
Temperature [K]	85
Detector technology	IMAGE PLATE
Collection date	2020-06-09
Detector	RIGAKU
Wavelength(s)	1.5870
Spacegroup name	P 1 21 1
Unit cell lengths	42.449, 61.100, 61.041
Unit cell angles	90.00, 97.65, 90.00

Refinement procedure

Resolution	42.990 - 2.289
R-factor	0.1896
Rwork	0.187
R-free	0.23710
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4qrc
RMSD bond length	0.003
RMSD bond angle	0.694
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.990	2.371
High resolution limit [Å]	2.289	2.289
Rmerge	0.092	0.244
Rmeas	0.112
Number of reflections	13012	930
<I/σ(I)>	7.87	2.87
Completeness [%]	92.2	66.1
Redundancy	2.7	1.9
CC(1/2)	0.990	0.876

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.5	277.15	0.1 M Bis-Tris (pH 4.5), 0.2 M Li2SO4, 18 % PEG 3350