7F1E

Structure of METTL6 bound with SAM

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-10-24
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9792
Spacegroup name	P 43
Unit cell lengths	83.457, 83.457, 129.686
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	26.857 - 2.589
R-factor	0.1934
Rwork	0.191
R-free	0.22650
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4nec
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	26.857	2.630
High resolution limit [Å]	2.589	2.589
Rmerge	0.094	1.056
Number of reflections	41739	870
<I/σ(I)>	20.8
Completeness [%]	99.9
Redundancy	12.5
CC(1/2)	0.992	0.699

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	0.1M Sodium acetate pH5.2, 2.0M sodium formate