7F0S

A crystal structure of alphavirus nonstructural protein 4 (nsP4) reveals an intrinsically 1dynamic RNA-dependent RNA polymerase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE TPS 05A
Synchrotron site	NSRRC
Beamline	TPS 05A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-12-20
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.946
Spacegroup name	P 21 21 21
Unit cell lengths	64.837, 68.349, 99.646
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	27.130 - 2.600
R-factor	0.2664
Rwork	0.265
R-free	0.28840
Structure solution method	SAD
RMSD bond length	0.006
RMSD bond angle	0.976
Data reduction software	XDS
Data scaling software	XDS
Phasing software	CRANK2
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.360
High resolution limit [Å]	2.300	2.300
Rmerge	0.064	4.800
Number of reflections	37774
<I/σ(I)>	12.04	0.36
Completeness [%]	99.8	98.4
Redundancy	5.69	5.69

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.1M HEPES/TRIS, 24% PEG 3350, 0.2M Ammonium Acetate, 0.3% TMAO