7EYI

Crystal structure of ZBTB7A in complex with gamma-globin -200 sequence element with C-194A mutation

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-12-05
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.979
Spacegroup name	P 43 21 2
Unit cell lengths	67.440, 67.440, 228.705
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.720 - 2.401
R-factor	0.2244
Rwork	0.222
R-free	0.26670
Structure solution method	SAD
Data reduction software	HKL-2000
Data scaling software	SCALA
Phasing software	PHENIX
Refinement software	PHENIX (1.16)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	2.440
High resolution limit [Å]	2.400	2.400
Rmerge	0.094	1.382
Number of reflections	19866	1029
<I/σ(I)>	36.9
Completeness [%]	99.1
Redundancy	25.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	0.1 M MIB buffer,pH 7.0, 25% (w/v) polyethylene glycol (PEG) 1500