7EYC
Crystal structure of Tau and acetylated tau peptide antigen
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 7A (6B, 6C1) |
Synchrotron site | PAL/PLS |
Beamline | 7A (6B, 6C1) |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2020-09-28 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 0.9730 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 76.599, 83.862, 134.371 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 36.810 - 2.490 |
R-factor | 0.1948 |
Rwork | 0.192 |
R-free | 0.24520 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4lex |
RMSD bond length | 0.011 |
RMSD bond angle | 1.505 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.540 |
High resolution limit [Å] | 2.489 | 6.780 | 2.500 |
Rmerge | 0.075 | 0.047 | 0.422 |
Rmeas | 0.083 | 0.052 | 0.464 |
Rpim | 0.035 | 0.023 | 0.191 |
Number of reflections | 30917 | 1682 | 1521 |
<I/σ(I)> | 13 | ||
Completeness [%] | 99.9 | 99.1 | 99.9 |
Redundancy | 5.5 | 4.8 | 5.6 |
CC(1/2) | 0.997 | 0.916 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 291 | 70% (+/-)-2-Methyl-2,4-pentanediol, 0.1 M HEPES, pH 7.5, 10 mM Calcium chloride |