7EX6
Crystal structure of Ebinur Lake virus cap snatching endonuclease in complex with inhibitor 11
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-01-14 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.9792 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 70.852, 41.667, 145.276 |
Unit cell angles | 90.00, 101.95, 90.00 |
Refinement procedure
Resolution | 47.380 - 2.180 |
R-factor | 0.2228 |
Rwork | 0.220 |
R-free | 0.27710 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2xi5 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.19_4080) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.260 |
High resolution limit [Å] | 2.180 | 4.700 | 2.180 |
Rmerge | 0.098 | 0.054 | 0.422 |
Rmeas | 0.121 | 0.068 | 0.517 |
Rpim | 0.071 | 0.041 | 0.295 |
Total number of observations | 112636 | ||
Number of reflections | 41653 | 3647 | 4254 |
<I/σ(I)> | 6.8 | ||
Completeness [%] | 95.2 | 79.8 | 99 |
Redundancy | 2.7 | 2.4 | 2.8 |
CC(1/2) | 0.995 | 0.819 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | EVAPORATION | 5.1 | 289 | PEG 3350 |