7EWW

Crystal structure of Ebinur Lake virus cap snatching endonuclease (N52A mutant)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-07-19
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9792
Spacegroup name	P 1 21 1
Unit cell lengths	39.988, 70.800, 70.272
Unit cell angles	90.00, 89.48, 90.00

Refinement procedure

Resolution	19.990 - 2.340
R-factor	0.2102
Rwork	0.208
R-free	0.24610
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2xi5
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.19_4080)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.420
High resolution limit [Å]	2.340	5.040	2.340
Rmerge	0.088	0.059	0.599
Rmeas	0.100	0.067	0.681
Rpim	0.047	0.031	0.315
Total number of observations	65861
Number of reflections	15597	1384	1617
<I/σ(I)>	8.9
Completeness [%]	94.1	81.7	97.5
Redundancy	4.2	4.2	4.3
CC(1/2)		0.996	0.751

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	5.3	289	PEG 3350