7EPM

human LDHC complexed with NAD+ and ethylamino acetic acid

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-10-22
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9789
Spacegroup name	P 61 2 2
Unit cell lengths	177.891, 177.891, 179.210
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	44.470 - 3.000
R-factor	0.2044
Rwork	0.203
R-free	0.22650
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1i10
RMSD bond length	0.011
RMSD bond angle	1.188
Data reduction software	HKL-3000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.070
High resolution limit [Å]	2.998	2.998
Number of reflections	34121	2229
<I/σ(I)>	15
Completeness [%]	100.0
Redundancy	21.3
CC(1/2)	0.987	0.602

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	0.1 M sodium chloride, 0.1 M HEPES pH 7.5, 1.6 M ammonium sulfate