7EOD
MITF HLHLZ Delta AKE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-07-07 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97852 |
| Spacegroup name | P 1 |
| Unit cell lengths | 33.020, 52.880, 55.470 |
| Unit cell angles | 82.64, 85.49, 71.85 |
Refinement procedure
| Resolution | 39.160 - 1.900 |
| R-factor | 0.1907 |
| Rwork | 0.188 |
| R-free | 0.23790 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7d8r |
| Data reduction software | DIALS |
| Data scaling software | Aimless (0.7.4) |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 40.000 | 40.000 | 1.940 |
| High resolution limit [Å] | 1.900 | 9.110 | 1.900 |
| Rmerge | 0.074 | 0.021 | 0.684 |
| Rmeas | 0.087 | 0.024 | 0.804 |
| Rpim | 0.045 | 0.012 | 0.419 |
| Number of reflections | 49812 | 235 | 1705 |
| <I/σ(I)> | 11.7 | ||
| Completeness [%] | 93.9 | 92.9 | 93.7 |
| Redundancy | 3.6 | 3.6 | 3.5 |
| CC(1/2) | 0.998 | 0.999 | 0.817 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 1.8 M Ammonium citrate pH 7.0 |
