7EJP

Crystal Structure of the Candida Glabrata Glycogen Debranching Enzyme (W470A) in complex with maltohexaose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2016-03-11
Detector	ADSC QUANTUM 315
Wavelength(s)	0.91769
Spacegroup name	C 1 2 1
Unit cell lengths	156.537, 199.828, 133.878
Unit cell angles	90.00, 100.78, 90.00

Refinement procedure

Resolution	38.440 - 3.100
R-factor	0.2369
Rwork	0.236
R-free	0.25560
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5d0f
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.440	3.150
High resolution limit [Å]	3.100	3.100
Rmerge	0.067
Number of reflections	73954	3680
<I/σ(I)>	15.74
Completeness [%]	99.8
Redundancy	3.8
CC(1/2)		0.427

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	20% PEG 5000 MME,0.1 M Tris pH7.0,5% Tasimate pH7.0, 3.5mM TCEP hydrochiloride