7EIC
Crystal structure of AF9 YEATS domain in complex with H4K5acK8ac peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B2 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-10-16 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 1 |
| Unit cell lengths | 30.367, 51.702, 56.273 |
| Unit cell angles | 89.30, 74.81, 89.87 |
Refinement procedure
| Resolution | 50.010 - 1.950 |
| R-factor | 0.17823 |
| Rwork | 0.177 |
| R-free | 0.20475 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4tmp |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.137 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.980 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Number of reflections | 22633 | 965 |
| <I/σ(I)> | 13.9 | 3.1 |
| Completeness [%] | 94.1 | |
| Redundancy | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 100 mM Bis-Tris buffer (pH 6.5) containing 100 mM ammonium acetate and 25% PEG3350 |
