7EF8
Crystal structure of mouse MUTYH in complex with DNA containing AP site analogue:8-oxoG (Form I)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NW12A |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-06-22 |
| Detector | DECTRIS PILATUS3 2M |
| Wavelength(s) | 1 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 74.274, 107.236, 156.432 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.890 - 2.450 |
| R-factor | 0.1988 |
| Rwork | 0.198 |
| R-free | 0.22060 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7ef9 |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.585 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.890 | 2.490 |
| High resolution limit [Å] | 2.450 | 2.450 |
| Rmerge | 0.094 | 0.619 |
| Number of reflections | 23331 | 1142 |
| <I/σ(I)> | 23.6 | |
| Completeness [%] | 99.8 | |
| Redundancy | 6.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | PEG3350, lithium sulfate, ammonium sulfate, Bis-Tris |
