7E9V
The Crystal Structure of human UMP-CMP kinase from Biortus.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CLSI BEAMLINE 08ID-1 |
Synchrotron site | CLSI |
Beamline | 08ID-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-08-27 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.97949 |
Spacegroup name | P 65 2 2 |
Unit cell lengths | 61.926, 61.926, 225.397 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 48.473 - 2.100 |
Rwork | 0.205 |
R-free | 0.24970 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1tev |
RMSD bond length | 0.009 |
RMSD bond angle | 1.636 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.473 | 2.160 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.075 | 0.937 |
Number of reflections | 15960 | 1267 |
<I/σ(I)> | 24 | |
Completeness [%] | 100.0 | |
Redundancy | 18.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 2.5M (NH4)2SO4, 0.1M Tris pH8.5 |