7E9K

Crystal Structure of POMGNT2 in complex with UDP and mono-mannosyl peptide (379Man long peptide)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P14 (MX2)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-09-19
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9763
Spacegroup name	P 1 21 1
Unit cell lengths	86.150, 104.401, 148.639
Unit cell angles	90.00, 90.10, 90.00

Refinement procedure

Resolution	49.550 - 2.050
R-factor	0.204
Rwork	0.202
R-free	0.24070
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	D_1300018733
RMSD bond length	0.003
RMSD bond angle	0.543
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.570	2.090
High resolution limit [Å]	2.050	2.050
Rmerge	0.070	0.801
Rpim	0.044	0.600
Number of reflections	163077	7958
<I/σ(I)>	10.8	1.6
Completeness [%]	99.0	99
Redundancy	3.5
CC(1/2)	0.999	0.761

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	0.1M Tris-HCl (pH 8.0 or 8.5), 12-16% PEG 8000, 1% tacsimate (pH 7.0)