7E5A

interferon-inducible anti-viral protein R356A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-01-11
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.978
Spacegroup name	P 32 2 1
Unit cell lengths	114.260, 114.260, 168.490
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	49.480 - 2.500
R-factor	0.2131
Rwork	0.211
R-free	0.24990
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1f5n
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.540
High resolution limit [Å]	2.500	6.780	2.500
Rmerge	0.123	0.075	1.294
Rmeas	0.131	0.081	1.376
Rpim	0.041	0.028	0.455
Total number of observations	401695
Number of reflections	44229	2310	2199
<I/σ(I)>	5.5
Completeness [%]	98.7	95.5	98.6
Redundancy	9.1	8.2	7.9
CC(1/2)		0.983	0.639

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICROBATCH		289.15	ammonium sulfate, HEPES pH 7.5, polyethylene glycol 3350