7E58
interferon-inducible anti-viral protein 2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL18U1 |
Synchrotron site | SSRF |
Beamline | BL18U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-01-02 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.978 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 42.207, 124.161, 355.722 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.030 - 2.600 |
R-factor | 0.2555 |
Rwork | 0.255 |
R-free | 0.27150 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1f5n |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 120.000 | 120.000 | 2.640 |
High resolution limit [Å] | 2.600 | 7.060 | 2.600 |
Rmerge | 0.151 | 0.059 | 1.325 |
Rmeas | 0.162 | 0.063 | 1.421 |
Rpim | 0.057 | 0.021 | 0.494 |
Total number of observations | 414059 | ||
Number of reflections | 55672 | 3315 | 2117 |
<I/σ(I)> | 4.3 | ||
Completeness [%] | 92.4 | 99.4 | 70.8 |
Redundancy | 7.4 | 8.6 | 7.2 |
CC(1/2) | 0.998 | 0.591 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | MICROBATCH | 289.15 | 2-propanol, sodium citrate, tribasic dihydrate pH 5.0, polyethylene glycol 10,000 |