7E53

Crystal structure of sfGFP complexed with the nanobody nb2 at 2.2 Angstron resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-11-09
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97875
Spacegroup name	C 2 2 21
Unit cell lengths	64.207, 123.991, 108.876
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.810 - 2.210
R-factor	0.2065
Rwork	0.204
R-free	0.25830
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2b3p 6itq
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.18_3861)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.000	30.000	2.280
High resolution limit [Å]	2.200	4.730	2.200
Rmerge	0.258	0.246	0.467
Rmeas	0.273	0.259	0.517
Rpim	0.086	0.077	0.212
Total number of observations	133097
Number of reflections	21347	2342	1943
<I/σ(I)>	9.8
Completeness [%]	96.0	99.4	89.1
Redundancy	6.2	10	4.1
CC(1/2)		0.964	0.340

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	30% (w/v) Polyethylene glycol 400, 100mM CAPS/ Sodium hydroxide, pH 10.5