7E4Z
Crystal structure of tubulin in complex with Maytansinol
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-07-04 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97908 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 104.431, 156.635, 182.275 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 38.285 - 2.690 |
R-factor | 0.194 |
Rwork | 0.192 |
R-free | 0.22820 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4tv8 |
RMSD bond length | 0.002 |
RMSD bond angle | 0.646 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.8.4_1496) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 40.000 | 40.000 | 2.800 |
High resolution limit [Å] | 2.690 | 5.810 | 2.700 |
Rmerge | 0.133 | 0.057 | 0.723 |
Rmeas | 0.138 | 0.060 | 0.750 |
Rpim | 0.037 | 0.017 | 0.199 |
Number of reflections | 83413 | 8729 | 8212 |
<I/σ(I)> | 4.2 | ||
Completeness [%] | 100.0 | 100 | 100 |
Redundancy | 13.4 | 12.5 | 13.9 |
CC(1/2) | 0.998 | 0.939 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.7 | 293 | 6% poly(ethylene glycol) 4000, 8% glycerol, 0.1 M MES (pH 6.7), 30 mM CaCl2, 30 mM MgCl2 |