7E4W
Human Transcriptional Co-activator PC4 (C-terminal Domain) in space group P1211
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | RRCAT INDUS-2 BEAMLINE PX-BL21 |
| Synchrotron site | RRCAT INDUS-2 |
| Beamline | PX-BL21 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2019-04-02 |
| Detector | RAYONIX MX-225 |
| Wavelength(s) | 0.97947 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 49.012, 166.844, 108.309 |
| Unit cell angles | 90.00, 93.27, 90.00 |
Refinement procedure
| Resolution | 108.130 - 2.900 |
| R-factor | 0.2301 |
| Rwork | 0.228 |
| R-free | 0.27760 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1pcf |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.534 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 108.130 | 3.000 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Number of reflections | 38333 | 3798 |
| <I/σ(I)> | 7.7 | 1.41 |
| Completeness [%] | 99.5 | 99.84 |
| Redundancy | 3.8 | |
| CC(1/2) | 0.988 | 0.614 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 285 | 20% MPD, 150-200 MM NACL, Phosphate Buffer (PH 5-5.2) |
