7DY2
Crystal Structure of Cyanobacterial Circadian Clock Protein KaiC
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-12-03 |
| Detector | RAYONIX MX-225 |
| Wavelength(s) | 0.9 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 92.803, 206.600, 168.383 |
| Unit cell angles | 90.00, 94.69, 90.00 |
Refinement procedure
| Resolution | 49.240 - 3.040 |
| R-factor | 0.2662 |
| Rwork | 0.263 |
| R-free | 0.32560 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2gbl |
| RMSD bond length | 0.002 |
| RMSD bond angle | 1.245 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0253) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.150 |
| High resolution limit [Å] | 3.038 | 3.038 |
| Rmerge | 0.133 | 0.899 |
| Number of reflections | 120308 | 12016 |
| <I/σ(I)> | 15.6 | |
| Completeness [%] | 99.9 | |
| Redundancy | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 5 | 313 | Acetatic acid, Sodium formate |
