7DW7

Crystal Structure of N1051A mutant of Formylglycinamidine Synthetase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	Cu FINE FOCUS
Temperature [K]	120
Detector technology	IMAGE PLATE
Collection date	2019-10-25
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.587
Spacegroup name	P 65
Unit cell lengths	146.626, 146.626, 141.022
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	47.990 - 1.800
R-factor	0.1345
Rwork	0.133
R-free	0.16250
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1t3t
RMSD bond length	0.023
RMSD bond angle	2.281
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0135)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.830
High resolution limit [Å]	1.800	1.800
Rpim	0.023
Number of reflections	158641	7938
<I/σ(I)>	34.5
Completeness [%]	100.0
Redundancy	8
CC(1/2)		0.931

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	2M Ammonium Sulphate