7DVD

The crystal structure of p53 DNA binding domain and PUMA complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	100
Detector technology	CCD
Collection date	2019-07-26
Detector	ADSC QUANTUM 270
Wavelength(s)	0.979
Spacegroup name	P 1
Unit cell lengths	68.612, 73.411, 84.408
Unit cell angles	89.94, 89.82, 90.00

Refinement procedure

Resolution	26.280 - 2.590
R-factor	0.2726
Rwork	0.270
R-free	0.31770
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2wxr
RMSD bond length	0.009
RMSD bond angle	1.143
Data reduction software	HKL-2000
Data scaling software	Coot
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.635
High resolution limit [Å]	2.590	2.590
Rmerge		0.323
Number of reflections	62203	2327
<I/σ(I)>	13.506
Completeness [%]	99.1
Redundancy	3.7
CC(1/2)	0.915

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293.15	0.1 M Tris (pH 7.0) 20% (w/w) PEG-200 MME