7DSY

Crystal Structure of RNase L in complex with KM05073

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	CCD
Collection date	2020-01-16
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9792
Spacegroup name	P 21 21 21
Unit cell lengths	59.320, 111.050, 262.830
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	31.504 - 2.651
R-factor	0.2024
Rwork	0.199
R-free	0.26990
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6m11
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	31.504	2.750
High resolution limit [Å]	2.650	2.650
Rmerge	0.065	0.947
Number of reflections	96516	4987
<I/σ(I)>	1.27
Completeness [%]	99.5
Redundancy	6.5
CC(1/2)	0.999

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	20% PEG4000, 100 mM Tris, 150 mM (NH4)2SO4, 2mM DTT, pH 7.5