7DSA
Crystal structure of actin capping protein in complex with V-1 (space group P62)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | AichiSR BEAMLINE BL2S1 |
Synchrotron site | AichiSR |
Beamline | BL2S1 |
Temperature [K] | 90 |
Detector technology | CCD |
Collection date | 2020-11-26 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 1.12 |
Spacegroup name | P 62 |
Unit cell lengths | 160.293, 160.293, 51.999 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 46.270 - 2.800 |
R-factor | 0.215 |
Rwork | 0.213 |
R-free | 0.25370 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3aaa |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | MOLREP |
Refinement software | PHENIX (1.18) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 46.270 | 46.270 | 2.970 |
High resolution limit [Å] | 2.800 | 8.280 | 2.800 |
Rmerge | 0.266 | 0.042 | 1.366 |
Rmeas | 0.279 | 0.044 | 1.433 |
Total number of observations | 212512 | ||
Number of reflections | 19202 | 791 | 3061 |
<I/σ(I)> | 11.71 | 45.9 | 2.1 |
Completeness [%] | 100.0 | 99.4 | 100 |
Redundancy | 11.067 | 10.024 | 10.97 |
CC(1/2) | 0.993 | 1.000 | 0.705 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 20% (w/v) PEG 3350, 0.2M Magnesium Acetate |