7DIE
Crystal structure of M. penetrans Ferritin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-07-17 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.979 |
| Spacegroup name | I 4 |
| Unit cell lengths | 151.334, 151.334, 125.213 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.856 - 1.900 |
| R-factor | 0.1536 |
| Rwork | 0.153 |
| R-free | 0.17720 |
| Structure solution method | SAD |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | AutoSol |
| Refinement software | PHENIX (1.15.2_3472) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.930 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmeas | 0.108 | 0.463 |
| Rpim | 0.041 | 0.174 |
| Number of reflections | 110615 | 5458 |
| <I/σ(I)> | 30.79 | 3.875 |
| Completeness [%] | 99.7 | |
| Redundancy | 6.9 | |
| CC(1/2) | 0.992 | 0.927 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 289 | 0.2 M ammonium acetate, 0.1 M Tris-HCl (pH 8.5), 10-15% (w/v) MPD |
