7DFC

Crystal of Arrestin2-V2Rpp-3-Fab30 complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	CCD
Collection date	2017-06-29
Detector	ADSC QUANTUM 315
Wavelength(s)	1.0500
Spacegroup name	I 21 21 21
Unit cell lengths	116.137, 122.653, 144.064
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.690 - 2.490
R-factor	0.2315
Rwork	0.229
R-free	0.28400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4jqi
RMSD bond length	0.009
RMSD bond angle	1.159
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.690	2.550
High resolution limit [Å]	2.450	2.450
Rmerge	0.062	0.959
Rpim	0.025	0.548
Number of reflections	38166	4165
<I/σ(I)>	18.5
Completeness [%]	99.6
Redundancy	7.2
CC(1/2)	0.999	0.850

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	7.5	289	15% PEG 3350, 0.1M HEPES, 0.2mM DMSO