7DBK
Crystal structure of human LDHB in complex with NADH
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-02-20 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 1 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 232.477, 84.173, 156.210 |
| Unit cell angles | 90.00, 120.76, 90.00 |
Refinement procedure
| Resolution | 49.993 - 1.802 |
| Rwork | 0.170 |
| R-free | 0.20340 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1i0z |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.548 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0266) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.830 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rpim | 0.042 | 0.324 |
| Number of reflections | 237609 | 11881 |
| <I/σ(I)> | 19.8 | 2.1 |
| Completeness [%] | 99.6 | 100 |
| Redundancy | 3.4 | |
| CC(1/2) | 0.994 | 0.736 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | PEG 3350, ammonium acetate, HEPES |
