7D9N

Crystal structure of a non-canonic progeria mutation S143F at lamin A/C and its structural implication to the premature aging

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	298
Detector technology	PIXEL
Collection date	2020-08-10
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.97942
Spacegroup name	I 41 2 2
Unit cell lengths	123.808, 123.808, 458.545
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.100 - 3.700
R-factor	0.2892
Rwork	0.287
R-free	0.30430
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6jlb
RMSD bond length	0.003
RMSD bond angle	0.563
Data reduction software	DENZO
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((1.19.2_4158: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	4.070
High resolution limit [Å]	3.700	4.000
Number of reflections	16492	14820
<I/σ(I)>	24.09
Completeness [%]	96.8
Redundancy	10.4
CC(1/2)	0.996	0.699

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		287	0.2 M Tris-Hcl pH8 0.1 M Sodium citrate 18% PEG 400